| 2. | Trial 2 , effect of supplemental copper of different type on nutrition and specific immunity of mice - ii the grouping of trialt animal was the same as trial 1 , at the first day , second day , third day , one mouse was injected with pha brine fluid for 10mg / kg avoirdupois after weighing in the same time in each repeat , following the 7d , 14d , 21d , 8d feeding period , after weighing , blood was made , wrigh - giemsa coloration , counting the number of lymphocyte female cell and overage lymphocyte , index of immune organ , copper concentration in liver and spleen 试验二,不同形式铜对小鼠营养与特异性免疫功能的作用-试验动物分组同试验一,进入正式试验期后,在每周第1天、 2天、 3天同一时间每重复选取1只小鼠,称重后每天按10mg kg体重肌肉注射一次植物血凝素生理盐水溶液,并于试验第7天、 14天、 21天、 28天称重后尾尖取血,姬姆萨-瑞氏染色,计算t淋巴细胞转化率,计算免疫器官指数,测定肝脏、脾脏铜含量。 |